the regenerative effect of bone marrow-derived stem cells in spermatogenesis of infertile hamster
نویسندگان
چکیده
background infertility is a serious social problem in advanced nations, with male factor in half of all cases of infertility. this study was conducted to determine the regenerative effect of bone marrow-derived stem cells in spermatogenesis of infertile hamster. methods twelve adult male hamsters were equally divided into azoospermic and control groups. busulfan was intraperitoneally used for induction of azoospermia, while the right testis was treated with bone marrow-derived stem cells (106 bm-scs), labeled with sterile trypan blue, 35 days after busulfan injection. the left testis served as positive control for azoospermia. sixty days after cell transplantation, the animals were euthanized and both testes were removed and evaluated histologically. results bm-scs were spindle-shaped, adherent to the culture flasks and had positive expression of cd29 and cd73 and negative expression of cd45. alcian blue staining confirmed differentiation of bm-scs into chondrocytes. karyotyping denoted to stability of chromosomes. treatment with busulfan in seminiferous tubules resulted into distruption of spermatogenesis. after two months in busulfan treatment group, seminiferous tubular atrophy and germinal epitheliums degenerations were noticed with no spermatozoa in epididymis. after treatment of busulfan group with bm-scs, spermatogonia, primary spermatocytes, spermatids and sperms were present in seminiferous tubules. conclusion as cell transplantation in seminiferous tubules resulted into a rapid repair of pathological changes, bm-scs can be recommended an effective treatment measure in azoospermia. it seems that more studies are necessary to confirm the use of this technique in treatment of azoospermia and infertility in human.
منابع مشابه
The Regenerative Effect of Bone Marrow-Derived Stem Cells in Spermatogenesis of Infertile Hamster
BACKGROUND Infertility is a serious social problem in advanced nations, with male factor in half of all cases of infertility. This study was conducted to determine the regenerative effect of bone marrow-derived stem cells in spermatogenesis of infertile hamster. METHODS Twelve adult male hamsters were equally divided into azoospermic and control groups. Busulfan was intraperitoneally used fo...
متن کاملThe Regenerative Effect of Bone Marrow-Derived Stem Cells in Spermatogenesis of Infertile Hamster
BACKGROUND Infertility is a serious social problem in advanced nations, with male factor in half of all cases of infertility. This study was conducted to determine the regenerative effect of bone marrow-derived stem cells in spermatogenesis of infertile hamster. METHODS Twelve adult male hamsters were equally divided into azoospermic and control groups. Busulfan was intraperitoneally used for...
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The present study determined the regenerative effect of bone marrow-derived stem cells (BMSCs) on thioacetamide (TA)-induced liver fibrosis in rats. A total of 30 male Wistar rats were randomly divided into sham control and treatment groups. The rats of the sham control group were subdivided into three groups and sampled on the 14th, 18th, and 20th weeks after fibrosis induction. The rats of th...
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The Healing Effect of Bone Marrow-Derived Stem Cells in Knee Osteoarthritis: A Case Report
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متن کاملInduction of Spermatogenesis by Bone Marrow-derived Mesenchymal Stem Cells in Busulfan-induced Azoospermia in Hamster
BACKGROUND Bone marrow-derived mesenchymal stem cells (BM-MSCs) have potential of differentiation and they secrete anti-inflammatory cytokines and growth factors which make them appropriate for cell therapy. AIM OF THE WORK Were to evaluate the healing effect of BM-MSCs transplantation on germinal cells of busulfan-induced azoospermic hamsters. MATERIAL AND METHODS In the present experiment...
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عنوان ژورنال:
world journal of plastic surgeryجلد ۶، شماره ۱، صفحات ۰-۰
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